pgp9 5  (Cedarlane)

Journal: iScience

Article Title: Rebalance of mitophagy by inhibiting LRRK2 improves colon alterations in an MPTP in vivo model

doi: 10.1016/j.isci.2024.110980

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Article Snippet: Rabbit polyclonal anti-PGP9.5 , Elabscience , E-AB-53097.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software

Journal: The Journal of comparative neurology

Article Title: Using tissue clearing and light sheet fluorescence microscopy for the three-dimensional analysis of sensory and sympathetic nerve endings that innervate bone and dental tissue of mice.

doi: 10.1002/cne.25582

Figure Lengend Snippet: FIGURE 1 Modified immunolabeling-enabled imaging of solvent-cleared organs (iDISCO/iDISCO+) tissue clearing protocol achieves transparency of mouse femur and dental tissue. (a) Schematic diagram of the tissue clearing procedure for the three-dimensional (3D) visualization of nerve fibers. (b and c) Whole mouse femur (b) and mandible (c) imaged before and after clearing (scale bars, 5 mm). (d and e) A 3D rendered surface of the bone marrow (d, scale bars, 1000 μm) and dental pulp (e, scale bars, 200 μm) tissue using manually drawn boundaries (20–30 μm apart) to view nerve fibers immunolabeled for protein gene product 9.5 (PGP9.5).

Article Snippet: Primary antibody Immunogen Manufacturing details Specificity/Characterization Dilution Calcitonin gene–related peptide (CGRP) Synthetic CGRP (rat) conjugated to KLH Sigma-Aldrich; rabbit polyclonal; Cat C8198, RRID: AB_259091 Lorenzo et al. (2008), Yen et al. (2006) 1:1000 Protein gene product 9.5 (PGP9.5) 15 amino synthetic peptide located near the C-terminus of human PGP9.5 (UCHL1) Cedarlane Laboratories; rabbit polyclonal; Cat CL7756AP, RRID: AB_2792979 Doran et al. (1983) 1:500 Tyrosine hydroxylase (TH) Denatured TH from rat pheochromocytoma Millipore; rabbit polyclonal; Cat AB152, RRID: AB_390204 Brown et al. (2011) 1:1000 Secondary antibody Manufacturing details Dilution Donkey anti-Rabbit Alexa Flour 647 Invitrogen; Cat A31573 1:1000 Abbreviation: KLH, keyhole limpet hemocyanin. antibodieswere replacedwithpreimmune serum fromthe samesource species, no staining was observed (Lorenzo et al., 2008; Yen et al., 2006).

Techniques: Modification, Immunolabeling, Imaging, Solvent

Journal: The Journal of comparative neurology

Article Title: Using tissue clearing and light sheet fluorescence microscopy for the three-dimensional analysis of sensory and sympathetic nerve endings that innervate bone and dental tissue of mice.

doi: 10.1002/cne.25582

Figure Lengend Snippet: FIGURE 2 Whole-tissue immunolabeling and three-dimensional (3D) fluorescence imaging to visualize protein gene product 9.5 (PGP9.5)-labeled nerve fibers and their endings in the mouse femur. (a) A simplified schematic representation of the neuronal distribution in the mouse femur. (b) A mosaic stitched, 3D reconstructed image of the bone marrow after surface rendering (scale bars, 1000 μm). (c) Insets show 300 μm maximum intensity z-projections of PGP9.5-labeled nerve fibers indicated in (b). Arrow indicates the entry site of a nerve bundle into the marrow cavity (scale bars, 1000 μm). (d) Insets show 200 μm z-projections of nerve fibers in the trabecular bone indicated in (b). Nerve bundles enter through the distal epiphysis (arrows), where they branch out and terminate in the marrow cavity (arrowheads). Dotted lines indicate the growth plate (scale bars, 500 μm). (e) 300 (left) and 200 μm (right) z-projections of nerve fibers imaged at higher power, showing PGP9.5-labeled axons wrapping around or running parallel to blood vessels (open arrows) and branching out and terminating near the endosteum (arrows) or in the

Article Snippet: Primary antibody Immunogen Manufacturing details Specificity/Characterization Dilution Calcitonin gene–related peptide (CGRP) Synthetic CGRP (rat) conjugated to KLH Sigma-Aldrich; rabbit polyclonal; Cat C8198, RRID: AB_259091 Lorenzo et al. (2008), Yen et al. (2006) 1:1000 Protein gene product 9.5 (PGP9.5) 15 amino synthetic peptide located near the C-terminus of human PGP9.5 (UCHL1) Cedarlane Laboratories; rabbit polyclonal; Cat CL7756AP, RRID: AB_2792979 Doran et al. (1983) 1:500 Tyrosine hydroxylase (TH) Denatured TH from rat pheochromocytoma Millipore; rabbit polyclonal; Cat AB152, RRID: AB_390204 Brown et al. (2011) 1:1000 Secondary antibody Manufacturing details Dilution Donkey anti-Rabbit Alexa Flour 647 Invitrogen; Cat A31573 1:1000 Abbreviation: KLH, keyhole limpet hemocyanin. antibodieswere replacedwithpreimmune serum fromthe samesource species, no staining was observed (Lorenzo et al., 2008; Yen et al., 2006).

Techniques: Immunolabeling, Fluorescence, Imaging, Labeling

Journal: The Journal of comparative neurology

Article Title: Using tissue clearing and light sheet fluorescence microscopy for the three-dimensional analysis of sensory and sympathetic nerve endings that innervate bone and dental tissue of mice.

doi: 10.1002/cne.25582

Figure Lengend Snippet: FIGURE 5 Whole-tissue immunolabeling and three-dimensional (3D) fluorescence imaging to visualize protein gene product 9.5 (PGP9.5)-labeled nerve fibers and their endings in the mouse mandible and surrounding tissues. (a) A simplified schematic representation of the neuronal distribution in the mouse mandible. (b) An 800 μm maximum intensity z-projection showing the distribution of inferior alveolar nerve branches through the mandible (scale bar, 500 μm). (c) A 3D-reconstructed image of the dental pulp after surface rendering (scale bar, 500 μm). (d) Schematic representation indicating the location from which e-j were sampled and viewed. (e) A 400 μm z-projection showing a bundle of nerve fibers that entered the molar through the apical foramen (arrows) and an accessory canal (arrowhead) (scale bar, 100 μm). (f) A 150 μm z-projection showing nerve fibers running through the mandibular alveolar bone, including the marrow, and nerve terminal endings in the periodontal ligament (arrowheads). Arrows indicate PGP9.5-labeled cells in the alveolar bone (scale bar, 100 μm). (g) An 80 μm z-projection showing nerve fibers running through the mandibular alveolar bone, including the marrow, and complex Ruffini-like endings in the periodontal ligament (open arrow)

Article Snippet: Primary antibody Immunogen Manufacturing details Specificity/Characterization Dilution Calcitonin gene–related peptide (CGRP) Synthetic CGRP (rat) conjugated to KLH Sigma-Aldrich; rabbit polyclonal; Cat C8198, RRID: AB_259091 Lorenzo et al. (2008), Yen et al. (2006) 1:1000 Protein gene product 9.5 (PGP9.5) 15 amino synthetic peptide located near the C-terminus of human PGP9.5 (UCHL1) Cedarlane Laboratories; rabbit polyclonal; Cat CL7756AP, RRID: AB_2792979 Doran et al. (1983) 1:500 Tyrosine hydroxylase (TH) Denatured TH from rat pheochromocytoma Millipore; rabbit polyclonal; Cat AB152, RRID: AB_390204 Brown et al. (2011) 1:1000 Secondary antibody Manufacturing details Dilution Donkey anti-Rabbit Alexa Flour 647 Invitrogen; Cat A31573 1:1000 Abbreviation: KLH, keyhole limpet hemocyanin. antibodieswere replacedwithpreimmune serum fromthe samesource species, no staining was observed (Lorenzo et al., 2008; Yen et al., 2006).

Techniques: Immunolabeling, Fluorescence, Imaging, Labeling